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<a href=https://bestcialis20mg.com/>buy cialis with paypal</a> Equivalent amounts of protein from whole cell lysates were mixed with 4 Laemmli s buffer, boiled for 5 minutes at 97 C, separated by SDS polyacrylamide 10 gel electrophoresis Thermo Fisher Scientific, transferred to nitrocellulose membranes GE Healthcare, Chicago, IL and probed with the antibodies described above